Method of the Year 2014 – CRISPR/Cas9 genome editing
Happy New Year, dear readers! Every year we are picking the “Method of the Year“. The methods in this contest should be relatively new (at least for human applications) and have huge potential impact...
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More on tissue clearing techniques
I’ve written about CLARITY tissue clearing techniques last year. What I did not mention is that there are so many of other tissue clearing techniques right now. The recent advances in these techniques...
View ArticleOff-the-shelf universal molecular beacon
Molecular beacon (MB) is a new tool to isolate cells, based on intracellular genetic marker. Specific molecular beacons were designed for positive selection of particular cell types. The next step in...
View ArticleCell viability assessment for whole bioengineered organ and 3D tissues
Whole organ bioengineering by recellularization of decellularized matrix is a new promising technology, which aims to address the problem of organs shortage in transplantation. Because bioengineered...
View ArticlemiRNA molecular switches for purification of pluripotent stem cell-derived...
Recently, Japanese researchers described a new methodology for purification of iPS/ES cell-derived tissue-specific progenitors by genetic markers. Unlike previous molecular beacon-based approaches,...
View ArticleNiche conditioning for stem cell engraftment
As you may know from this blog, host conditioning is required for successful engraftment of hematopoietic stem cells in BMT models. Conditioning allows to prep bone marrow niche for donor stem cells...
View ArticleThe ethics of in vitro mini-brains and mini-embryos
I’m trying to avoid ethical and legal issues, surrounding stem cell research, but some of them are really intriguing. Today, I’d like to highlight the discussion about ethical considerations in...
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Method of the Year 2015 – Optogenetic control of cell fate
Happy New Year, dear readers! Every year we are picking the “Method of the Year“. The best method of the year should be relatively new (at least for human applications) and have huge potential impact...
View ArticleMogrify – a new computational tool for direct reprogramming
This week, international group of researchers described a new algorithm for identification of transcription factors, used in direct cell reprogramming. This is freely available computational tool,...
View ArticleUsing aptamers for cell isolation
Aptamers are single-stranded RNA or DNA oligonucleotides, which could be screened and synthesized for specific target (including any cell type), using systematic evolution of ligands by exponential...
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